DNA Assembly and cloning kit
A Next Generation Cloning and DNA assembly kit that is Efficient, Precise , Flexible and Simply easy to use !
Simply Seamless is a proprietary enzyme mix from Synovance.
Simply Seamless facilitates the assembly of DNA fragments via homologous terminal regions without leaving any extra sequences behind (Seamless Assembly). Cloning is now a simple incubation step followed by classical transformation into chemically competent E. coli cells, yielding clones the next day.
Our Simply Seamless Cloning and DNA Assembly Kit enables:
– The simultaneous and directional cloning of 1 to 4 DNA fragments.
– DNA fragments can be either synthetic DNA purchased from a commercial provider or PCR fragments.
– The assembly is completely sequence independent, no need to add or remove restriction sites.
– Can direct fragments into any location within any vector of your choice.
– The reaction is complete within 15-30 mins at 37°C.
Simply Seamless Kit is the choice for creating all of your DNA constructs!
Figure 1: Assembly of four 1kb PCR products. a) Diagram
illustrating the assembly of four 1 kb fragments containing 25 bp homologous overlap regions b) 1% Agarose gel comparing the two assembly reactions: 1. Simply Seamless, 2. Competitor. 100 ng of each PCR fragment was assembled in each reaction. The 4kb band is the fully assembled product.
Figure 2: Cloning of a single fragment into a
vector. a) 1% Agarose gel comparing the two assembly reactions: 1. Simply Seamless, 2. Competitor. 100 ng of a PCR fragment (3.8 kb) was cloned into a vector (2.9 kb) in each reaction. The 6.82 kb band is the fully assembled product. b) Graph comparing the number of transformants (CFUs/ml) from the two different reactions. 3 ul of each 10 ul reaction was transformed and plated on LB with ampicillin.
Efficiency: Several fragments can simultaneously be put together in a single reaction with extremely high fidelity.
Flexibility: Design and add almost any sequence you desire in any combination you need. Use your own vector tools or engineer completely new ones to fit your needs.
Precision: Engineer the exact sequence that you design for performing precise genome edits at any desired location on the genome.
Simple and Fast: Design of the 25 bp homologies is easy and the assembly reaction is fast. This frees you up to spend your time on other projects, increasing your lab’s productivity.
Assemble multiple fragments to precisely create large constructs that contain no unwanted sequences (scars) in a single highly efficient reaction. Save weeks of work by circumventing the need for restriction enzymes, de-phosphorylation, ligations, reaction clean-up, and loss of your precious DNA!
Simultaneously assemble complex biosynthetic pathways that consist of multiple DNA fragments in a single 30-minute reaction. Use any linearized vector of your choice, no restriction digestion or recombination sites are required or forbidden. Your assembled product can be used to insert, delete or exchange DNA segments in genome engineering projects.
Therapeutics / Diagnostics
Construct tools for protein production and engineering diagnostic kits. Using the sequence of your choice you can precisely piece together any construct that you design without any sequence constraints.
Synthetic Genomics offers endless possibilities for advancing both Research and Industrial activities. These projects will benefit from our Highly-Efficient and Affordable DNA Assembly technologies. Complex genetic programs can be re-wired and incorporated into genomes that have been optimized for efficiency and robustness from the “bottom up”.
We are team of experts in genetics, molecular biology, biochemistry, genomics, synthetic biology and industrial operations.
We offer a full range of services, from cloning, gene knock-outs, genomic integrations / mutations, library construction. Don’t hesitate, We Can Help! For more information, please send us email at email@example.com and we will get back to you within 24 hours.